The labile interactions of cyclic electron flow effector proteins.

ABSTRACT: The supramolecular organization of membrane proteins (MPs) is sensitive to environmental changes in photosynthetic organisms. Isolation of MP supercomplexes from the green algae Chlamydomonas reinhardtii, which are believed to contribute to cyclic electron flow (CEF) between the cytochrome b₆f complex (Cyt-b₆f) and photosystem I (PSI), proved difficult. We were unable to isolate a supercomplex containing both Cyt-b₆f and PSI because in our hands, most of Cyt-b₆f did not comigrate in sucrose density gradients, even upon using chemical cross-linkers or amphipol substitution of detergents. Assisted by independent affinity purification and MS approaches, we utilized disintegrating MP asse

SUBMITTER: Buchert F 

PROVIDER: S-EPMC6231120 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature