Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in a Complete Serum-free Condition.

ABSTRACT: Mesenchymal stem cells (MSC) have been derived from a variety of tissues, and cultured either in animal serum-containing (SC) or serum-free (SF) media. We have previously derived MSC from human embryonic stem cells via an intermediate trophoblast step (named EMSC), which also have immunosuppressive and therapeutic effects on animal models of autoimmune disease. To promote the clinical application of this new source of MSC, we report here EMSC derived and cultured in a SF medium MesenCult (SF-EMSC) in comparison with a SC medium (SC-EMSC). SF-EMSC derived in MesenCult also expressed typical MSC markers CD73, CD90, and CD105, and manifested multipotency to differentiate to osteocytes, chondrocytes, and adipocytes. Comparably, CD105⁺ cells reached 90% about one week slower in the S

SUBMITTER: Li E 

PROVIDER: S-EPMC6231213 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature