Mechanistic Insights Into the Cross-Feeding of <i>Ruminococcus gnavus</i> and <i>Ruminococcus bromii</i> on Host and Dietary Carbohydrates.

ABSTRACT: Dietary and host glycans shape the composition of the human gut microbiota with keystone carbohydrate-degrading species playing a critical role in maintaining the structure and function of gut microbial communities. Here, we focused on two major human gut symbionts, the mucin-degrader Ruminococcus gnavus ATCC 29149, and R. bromii L2-63, a keystone species for the degradation of resistant starch (RS) in human colon. Using anaerobic individual and co-cultures of R. bromii and R. gnavus grown on mucin or starch as sole carbon source, we showed that starch degradation by R. bromii supported the growth of R. gnavus whereas R. bromii did not benefit from mucin degradation by R. gnavus. Further we analyzed the growth (quantitative PCR), meta

SUBMITTER: Crost EH 

PROVIDER: S-EPMC6231298 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature