Long ncRNA Landscape in the Ileum of Treatment-Naive Early-Onset Crohn Disease.

ABSTRACT:

Background

Long noncoding RNAs (lncRNA) are key regulators of gene transcription and many show tissue-specific expression. We previously defined a novel inflammatory and metabolic ileal gene signature in treatment-naive pediatric Crohn disease (CD). We now extend our analyses to include potential regulatory lncRNA.

Methods

Using RNAseq, we systematically profiled lncRNAs and protein-coding gene expression in 177 ileal biopsies. Co-expression analysis was used to identify functions and tissue-specific expression. RNA in situ hybridization was used to validate expression. Real-time polymerase chain reaction was used to test lncRNA regulation by IL-1β in Caco-2 enterocytes.

Results

We characterize widespread dysregulation of 459 lncRNAs in the ileum of CD patients. Using

SUBMITTER: Haberman Y 

PROVIDER: S-EPMC6231367 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature