Sputum Moraxella catarrhalis strains exhibit diversity within and between COPD subjects.

ABSTRACT:

Purpose

Moraxella catarrhalis is implicated in the pathogenesis of some COPD exacerbations. We sought to investigate whether the M. catarrhalis strain is variable between COPD subjects; that an exacerbation is associated with acquisition of a new strain and that certain strains are more commonly associated with exacerbations.

Patients and methods

Sputum samples were collected at stable and exacerbation visits from COPD subjects from a single center as part of the COPDMAP consortium. Samples identified as M. catarrhalis positive by qPCR were recultured in liquid cultures grown to extract genomic DNA; underwent Illumina MiSeq and bacterial genome sequences were de novo assembled and Multi Locus Sequence Type (MLST) was determined.

Results

Thirty-fiv

SUBMITTER: George LM 

PROVIDER: S-EPMC6231509 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature