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Decoupling from yolk sac is required for extraembryonic tissue spreading in the scuttle fly Megaselia abdita.


ABSTRACT: Extraembryonic tissues contribute to animal development, which often entails spreading over embryo or yolk. Apart from changes in cell shape, the requirements for this tissue spreading are not well understood. Here, we analyze spreading of the extraembryonic serosa in the scuttle fly Megaselia abdita. The serosa forms from a columnar blastoderm anlage, becomes a squamous epithelium, and eventually spreads over the embryo proper. We describe the dynamics of this process in long-term, whole-embryo time-lapse recordings, demonstrating that free serosa spreading is preceded by a prolonged pause in tissue expansion. Closer examination of this pause reveals mechanical coupling to the underlying yolk sac, which is later released. We find mechanical coupling prolonged and serosa spreading impaired after knockdown of M. abdita Matrix metalloprotease 1. We conclude that tissue-tissue interactions provide a critical functional element to constrain spreading epithelia.

SUBMITTER: Caroti F 

PROVIDER: S-EPMC6231767 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Decoupling from yolk sac is required for extraembryonic tissue spreading in the scuttle fly <i>Megaselia abdita</i>.

Caroti Francesca F   González Avalos Everardo E   Noeske Viola V   González Avalos Paula P   Kromm Dimitri D   Wosch Maike M   Schütz Lucas L   Hufnagel Lars L   Lemke Steffen S  

eLife 20181030


Extraembryonic tissues contribute to animal development, which often entails spreading over embryo or yolk. Apart from changes in cell shape, the requirements for this tissue spreading are not well understood. Here, we analyze spreading of the extraembryonic serosa in the scuttle fly <i>Megaselia abdita</i>. The serosa forms from a columnar blastoderm anlage, becomes a squamous epithelium, and eventually spreads over the embryo proper. We describe the dynamics of this process in long-term, whole  ...[more]

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