Increased succinate receptor GPR91 involved in the pathogenesis of Mooren's ulcer.

ABSTRACT:

Aim

To investigate the expression of succinate receptor GPR91 and its pathogenic roles in Mooren's ulcer (MU).

Methods

Biopsy specimens were obtained from 7 patients with MU and 6 healthy donors. The expression of GPR91 in MU tissues was evaluated using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). Succinate was used to activate GPR91 signaling, and the effect of GPR91 on the expression of interleukin-1β (IL-1β), NLRP3, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-13 (MMP-13) in human peripheral blood mononuclear cells (PBMCs) was determined. The influence of GPR91 on the nuclear factor-κB (NF-κB) signaling in PBMCs was investigated by detecting the phosphorylation of p65. Moreover,

SUBMITTER: Li L 

PROVIDER: S-EPMC6232339 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature