Molecular Mass and Localization of α-1,3-Glucan in Cell Wall Control the Degree of Hyphal Aggregation in Liquid Culture of Aspergillus nidulans.

ABSTRACT: α-1,3-Glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two α-1,3-glucan synthase genes, agsA and agsB. We previously revealed that AgsB is a major α-1,3-glucan synthase in vegetative hyphae, but the function of AgsA remained unknown because of its low expression level and lack of phenotypic alteration upon gene disruption. To clarify the role of α-1,3-glucan in hyphal aggregation, we constructed strains overexpressing agsA (agsA^OE ) or agsB (agsB^OE ), in which the other α-1,3-glucan synthase gene was disrupted. In liquid culture, the wild-type and agsB^OE strains formed tightly aggregated hyphal pellets, whereas agsA^OE hyphae agg

SUBMITTER: Miyazawa K 

PROVIDER: S-EPMC6232457 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature