RNA polymerase II CTD interactome with 3' processing and termination factors in fission yeast and its impact on phosphate homeostasis.

ABSTRACT: The carboxy-terminal domain (CTD) code encrypted within the Y¹S²P³T⁴S⁵P⁶S⁷ heptad repeats of RNA polymerase II (Pol2) is deeply rooted in eukaryal biology. Key steps to deciphering the code are identifying the events in gene expression that are governed by individual "letters" and then defining a vocabulary of multiletter "words" and their meaning. Thr4 and Ser7 exert opposite effects on the fission yeast pho1 gene, expression of which is repressed under phosphate-replete conditions by transcription of an upstream flanking long noncoding RNA (lncRNA). Here we attribute the derepression of pho1 by a CTD-S7A mutation to precocious termination of lncRNA synthesis, an effect that is erased by muta

SUBMITTER: Sanchez AM 

PROVIDER: S-EPMC6233144 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature