Enhancing the production of cephalosporin C through modulating the autophagic process of Acremonium chrysogenum.

ABSTRACT:

Background

Autophagy is used for degradation of cellular components and nutrient recycling. Atg8 is one of the core proteins in autophagy and used as a marker for autophagic detection. However, the autophagy of filamentous fungi is poorly understood compared with that of Saccharomyces cerevisiae. Our previous study revealed that disruption of the autophagy related gene Acatg1 significantly enhanced cephalosporin C yield through reducing degradation of cephalosporin biosynthetic proteins in Acremonium chrysogenum, suggesting that modulation of autophagic process is one promising way to increase antibiotic production in A. chrysogenum.

Results

In this study, a S. cerevisiae ATG8 homologue gene Acatg8 was identified from A. chrysogenum. Acatg8 could complement the ATG8 mutation

SUBMITTER: Li H 

PROVIDER: S-EPMC6233533 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature