General control non-repressible 20 (GCN20) functions in root growth by modulating DNA damage repair in Arabidopsis.

ABSTRACT:

Background

Most ABC transporters are engaged in transport of various compounds, but its subfamily F lacks transmembrane domain essential for chemical transportation. Thus the function of subfamily F remains further elusive.

Results

Here, we identified General Control Non-Repressible 20 (GCN20), a member of subfamily F, as new factor for DNA damage repair in root growth. While gcn20-1 mutant had a short primary root with reduced meristem size and cell number, similar primary root lengths were assayed in both wild-type and GCN20::GCN20 gcn20-1 plants, indicating the involvement of GCN20 in root elongation. Further experiments with EdU incorporation and comet assay demonstrated that gcn20-1 displays increased cell cycle arrest at G2/M checkpoint and accumulates more damaged DNA

SUBMITTER: Han TT 

PROVIDER: S-EPMC6233562 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature