Development of novel EST-SSR markers in the macauba palm (Acrocomia aculeata) using transcriptome sequencing and cross-species transferability in Arecaceae species.

ABSTRACT:

Background

The macaúba palm is a novel feedstock for oil production suitable for multiple uses, including as biodiesel and in the food and cosmetic industries. As an efficient alternative, the macaúba palm has limited genomic resources, particularly expressed sequence tag (EST) markers. We report a comprehensive set of validated EST-simple sequence repeat (SSR) markers by using transcriptome sequencing, its application in genetic diversity analysis and cross transferability in other palm trees with environmental and economic importance.

Results

In this study, a total of 418 EST-SSRs were identified to be unique for one transcript and region; 232 EST-SSRs were selected, with trinucleotide repeats being the most frequent motif, representing 380 (90.9%), followed by composited

SUBMITTER: Bazzo BR 

PROVIDER: S-EPMC6233587 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature