A functional human motor unit platform engineered from human embryonic stem cells and immortalized skeletal myoblasts.

ABSTRACT:

Background

Although considerable research on neuromuscular junctions (NMJs) has been conducted, the prospect of in vivo NMJ studies is limited and these studies are challenging to implement. Therefore, there is a clear unmet need to develop a feasible, robust, and physiologically relevant in vitro NMJ model.

Objective

We aimed to establish a novel functional human NMJs platform, which is serum and neural complex media/neural growth factor-free, using human immortalized myoblasts and human embryonic stem cells (hESCs)-derived neural progenitor cells (NPCs) that can be used to understand the mechanisms of NMJ development and degeneration.

Methods

Immortalized human myoblasts were co-cultured with hESCs derived committed NPCs. Over the course of the 7 days myoblasts diff

SUBMITTER: Abd Al Samid M 

PROVIDER: S-EPMC6233953 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature