Ribosomal protein S27-like regulates autophagy via the β-TrCP-DEPTOR-mTORC1 axis.

ABSTRACT: RPS27L (Ribosomal protein S27-like), an evolutionarily conserved ribosomal protein, is a p53 target and a physiological p53 regulator. We previously reported that Rps27l disruption enhanced lymphomagenesis in Trp53^+/- mice by triggering genome instability and sensitized Trp53^+/- mice to radiation by blocking DNA damage response. Whether and how RPS27L modulates autophagy is totally unknown. Here we report that RPS27L silencing significantly induced autophagy in breast cancer MB231 and SK-BR3 cells harboring mutant p53. Mechanistically, RPS27L silencing remarkably inactivated mTORC1, a major negative autophagy regulator, but not mTORC2. Autophagy induction and mTORC1 inactivation was also observed in MEFs with Rps27l deletion. More specifically, R

SUBMITTER: Xiong X 

PROVIDER: S-EPMC6234217 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature