Evaluating the Information Content of Shallow Shotgun Metagenomics.

ABSTRACT: Although microbial communities are associated with human, environmental, plant, and animal health, there exists no cost-effective method for precisely characterizing species and genes in such communities. While deep whole-metagenome shotgun (WMS) sequencing provides high taxonomic and functional resolution, it is often prohibitively expensive for large-scale studies. The prevailing alternative, 16S rRNA gene amplicon (16S) sequencing, often does not resolve taxonomy past the genus level and provides only moderately accurate predictions of the functional profile; thus, there is currently no widely accepted approach to affordable, high-resolution, taxonomic, and functional microbiome analysis. To address this technology gap, we evaluated the information content of shallow shotgun sequencing

SUBMITTER: Hillmann B 

PROVIDER: S-EPMC6234283 | biostudies-literature | 2018 Nov-Dec

REPOSITORIES: biostudies-literature