Succession and persistence of microbial communities and antimicrobial resistance genes associated with International Space Station environmental surfaces.

ABSTRACT:

Background

The International Space Station (ISS) is an ideal test bed for studying the effects of microbial persistence and succession on a closed system during long space flight. Culture-based analyses, targeted gene-based amplicon sequencing (bacteriome, mycobiome, and resistome), and shotgun metagenomics approaches have previously been performed on ISS environmental sample sets using whole genome amplification (WGA). However, this is the first study reporting on the metagenomes sampled from ISS environmental surfaces without the use of WGA. Metagenome sequences generated from eight defined ISS environmental locations in three consecutive flights were analyzed to assess the succession and persistence of microbial communities, their antimicrobial resistance (AMR) profiles, and vir

SUBMITTER: Singh NK 

PROVIDER: S-EPMC6234677 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature