Aberrant localization of FOXJ1 correlates with the disease severity and comorbidities in patients with nasal polyps.

ABSTRACT:

Background

Upper airway inflammatory diseases are associated with abnormal expression of nasal epithelial forkhead-box J1 (FOXJ1) which regulates motile cilia formation. We sought to investigate whether aberrant FOXJ1 localizations correlate with the disease severity and the co-existence of allergic rhinitis (AR) or asthma in patients with nasal polyps (NPs).

Methods

We elucidated localization patterns of FOXJ1 by performing immunofluorescence assays in nasal specimens and cytospin samples from controls and patients with NPs. We also assayed mRNA expression levels of FOXJ1 by using quantitative real-time polymerase chain reaction. Four localization patterns [normal (N), intermediate (I), mislocalization (M), and absence (A)] were defined. A semi-quantitative scoring s

SUBMITTER: Peng Y 

PROVIDER: S-EPMC6234688 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature