A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells.

ABSTRACT: Ovarian granulosa cells (GC) are the major source of estradiol synthesis. Induced by the preovulatory luteinizing hormone (LH) surge, cells of the theca and, in particular, of the granulosa cell layer profoundly change their morphological, physiological, and molecular characteristics and form the progesterone-producing corpus luteum that is responsible for maintaining pregnancy. Cell culture models are essential tools to study the underlying regulatory mechanisms involved in the folliculo-luteal transformation. The presented protocol focuses on the isolation procedure and cryopreservation of bovine GC from small- to medium-sized follicles (< 6 mm). With this technique, a nearly pure population of GC can be obtained. The cryopreservation procedure greatly facilitates time management of the

SUBMITTER: Baufeld A 

PROVIDER: S-EPMC6235104 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature