An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing.

ABSTRACT: Binary transcription systems are powerful genetic tools widely used for visualizing and manipulating cell fate and gene expression in specific groups of cells or tissues in model organisms. These systems contain two components as separate transgenic lines. A driver line expresses a transcriptional activator under the control of tissue-specific promoters/enhancers, and a reporter/effector line harbors a target gene placed downstream to the binding site of the transcription activator. Animals harboring both components induce tissue-specific transactivation of a target gene expression. Precise spatiotemporal expression of the gene in targeted tissues is critical for unbiased interpretation of cell/gene activity. Therefore, developing a method for generating exclusive cell/tissue-specific driv

SUBMITTER: Du L 

PROVIDER: S-EPMC6235241 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature