Overexpression of BcHsfA1 transcription factor from Brassica campestris improved heat tolerance of transgenic tobacco.

ABSTRACT: Heat shock proteins (HSPs) are a type of conserved molecular chaperone. They exist extensively in plants and greatly contribute to their survival under heat stress. The transcriptional regulation factor heat shock factor (HSF) is thought to regulate the expression of Hsps. In this study, a novel gene designated BcHsfA1 was cloned and characterized from Brassica campestris. Bioinformatic analysis implied that BcHsfA1 belongs to the HsfA gene family and is most closely related to HsfA1 from other plants. Constitutive overexpression of BcHsfA1 significantly improved heat tolerance of tobacco seedlings by affecting physiological and biochemical processes. Moreover, the chlorophyll content of transgenic tobacco plants was significantly increased compared with wild type after heat stress, as wer

SUBMITTER: Zhu X 

PROVIDER: S-EPMC6235349 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature