Quantifying Intermembrane Distances with Serial Image Dilations.

ABSTRACT: A recently-described extracellular nanodomain, termed the perinexus, has been implicated in ephaptic coupling, which is an alternative mechanism for electrical conduction between cardiomyocytes. The current method for quantifying this space by manual segmentation is slow and has low spatial resolution.We developed an algorithm that uses serial image dilations of a binary outline to count the number of pixels between two opposing 2 dimensional edges.This algorithm requires fewer man hours and has a higher spatial resolution than the manual method while preserving the reproducibility of the manual process.In fact, experienced and novice investigators were able to recapitulate the results of a previous study with this new algorithm.The algorithm is limited by the human input needed to manuall

SUBMITTER: Raisch T 

PROVIDER: S-EPMC6235350 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature