Effects of microcompartmentation on flux distribution and metabolic pools in <i>Chlamydomonas reinhardtii</i> chloroplasts.

ABSTRACT: Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco,

SUBMITTER: Kuken A 

PROVIDER: S-EPMC6235561 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature