Polydopamine-assisted BMP-2 immobilization on titanium surface enhances the osteogenic potential of periodontal ligament stem cells via integrin-mediated cell-matrix adhesion.

ABSTRACT: A mussel-inspired polydopamine (PDA), resulting from the oxidative polymerization of dopamine, was reported to be an attractive substrate for advancing biomaterial applications. Thus, this study determined the osteoconductive/osteoinductive properties of titanium (Ti) surfaces coated with PDA and the facilitation of the PDA layer to immobilize bone morphogenetic protein-2 (BMP-2) on Ti substrates. The surface chemistry of PDA or PDA/BMP-2-coated Ti was confirmed by contact angle measurement, scanning electron microscopy (SEM), immunofluorescence staining, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). We verified the osteogenic potential of periodontal ligament stem cells (PDLSCs) cultured on the PDA or PDA/BMP-2-Ti surfaces. The osteogenic differentiation of th

SUBMITTER: Lee JS 

PROVIDER: S-EPMC6235778 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature