Increase of Ca_V3 channel activity induced by HVA β1b-subunit is not mediated by a physical interaction.

ABSTRACT:

Objective

Low voltage-activated (LVA) calcium channels are crucial for regulating oscillatory behavior in several types of neurons and other excitable cells. LVA channels dysfunction has been implicated in epilepsy, neuropathic pain, cancer, among other diseases. Unlike for High Voltage-Activated (HVA) channels, voltage-dependence and kinetics of currents carried by recombinant LVA, i.e., Ca_V3 channels, are quite similar to those observed in native currents. Therefore, whether these channels are regulated by HVA auxiliary subunits, remain controversial. Here, we used the α1-subunits of Ca_V3.1, Ca_V3.2, and Ca_V3.3 channels, together with HVA auxiliary β-subunits to perform electrophysiological, confocal microscopy and immunoprecipitation ex

SUBMITTER: Arteaga-Tlecuitl R 

PROVIDER: S-EPMC6236959 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature