Method for Biomonitoring DNA Adducts in Exfoliated Urinary Cells by Mass Spectrometry.

ABSTRACT: Tobacco smoking contributes to about 50% of the bladder-cancer (BC) cases in the United States. Some aromatic amines in tobacco smoke are bladder carcinogens; however, other causal agents of BC are uncertain. Exfoliated urinary cells (EUCs) are a promising noninvasive biospecimen to screen for DNA adducts of chemicals that damage the bladder genome, although the analysis of DNA adducts in EUCs is technically challenging because of the low number of EUCs and limiting quantity of cellular DNA. Moreover, EUCs and their DNA adducts must remain viable during the time of collection and storage of urine to develop robust screening methods. We employed RT4 cells, a well-differentiated transitional epithelial bladder cell line, as a cell-model system in urine to investigate cell viability and the c

SUBMITTER: Yun BH 

PROVIDER: S-EPMC6237078 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature