Defining hydrogel properties to instruct lineage- and cell-specific mesenchymal differentiation.

ABSTRACT: The maintenance and direction of stem cell lineage after implantation remains challenging for clinical translation. Aggregation and encapsulation into instructive biomaterials after preconditioning can bolster retention of differentiated phenotypes. Since these procedures do not depend on cell type or lineage, we hypothesized we could use a common, tunable platform to engineer formulations that retain and enhance multiple lineages from different cell populations. To test this, we varied alginate stiffness and adhesive ligand content, then encapsulated spheroids of varying cellularity. We used Design-of-Experiments to determine the effect of these parameters and their interactions on phenotype retention. The combination of parameters leading to maximal differentiation varied with lineage an

SUBMITTER: Hung BP 

PROVIDER: S-EPMC6237198 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature