Chromatin remodelers couple inchworm motion with twist-defect formation to slide nucleosomal DNA.

ABSTRACT: ATP-dependent chromatin remodelers are molecular machines that control genome organization by repositioning, ejecting, or editing nucleosomes, activities that confer them essential regulatory roles on gene expression and DNA replication. Here, we investigate the molecular mechanism of active nucleosome sliding by means of molecular dynamics simulations of the Snf2 remodeler translocase in complex with a nucleosome. During its inchworm motion driven by ATP consumption, the translocase overwrites the original nucleosome energy landscape via steric and electrostatic interactions to induce sliding of nucleosomal DNA unidirectionally. The sliding is initiated at the remodeler binding location via the generation of a pair of twist defects, which then spontaneously propagate to complete sliding t

SUBMITTER: Brandani GB 

PROVIDER: S-EPMC6237416 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature