Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential.

ABSTRACT: Hepatocellular carcinoma (HCC) is one of the most common causes for cancer-related death worldwide with rapidly increasing incidence and mortality rates. As for other types of cancers, also in HCC cancer stem cells (CSCs) are thought to be responsible for tumour initiation, progression and therapy failure. However, as rare subpopulations of tumour tissue, CSCs are difficult to isolate, thus making the development of suitable and reliable model systems necessary. In our study, we generated HepG2 subclones with enriched CSC potential by application of the spheroid formation method and subsequent single-cell cloning. Analyses in several 2D and 3D cell culture systems as well as a panel of functional assays both in vitro and in vivo revealed that the generated subclones displayed characteristi

SUBMITTER: Muenzner JK 

PROVIDER: S-EPMC6237557 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature