PML-RARα interferes with erythropoiesis by repressing LMO2 in acute promyelocytic leukaemia.

ABSTRACT: The PML-RARα fusion gene, generated by the t(15;17) chromosome translocation, is regarded as the initiating factor of acute promyelocytic leukaemia (APL). In addition to the well-known effects on blocking myeloid differentiation at the promyelocytic stage, promyelocytic leukaemia-retinoic acid receptor α (PML-RARα) has also been reported to interfere with multiple differentiation processes, including erythroid differentiation. However, the detailed molecular mechanism by which PML-RARα impairs erythropoiesis has not yet been fully addressed. By chromatin immunoprecipitation-PCR assay, we found that PML-RARα bound to the distal promoter region of LMO2 (LIM-only protein 2), a critical erythroid-specific transcription factor. Luciferase reporter assays and qRT-PCR results demonstrated that PM

SUBMITTER: Yang X 

PROVIDER: S-EPMC6237603 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature