Role of Temperate Bacteriophage ϕ20617 on Streptococcus thermophilus DSM 20617^T Autolysis and Biology.

ABSTRACT: Streptococcus thermophilus DSM 20167^T showed autolytic behavior when cultured in lactose- and sucrose-limited conditions. The amount of cell lysis induced was inversely related to the energetic status of the cells, as demonstrated by exposing cells to membrane-uncoupling and glycolysis inhibitors. Genome sequence analysis of strain DSM 20617^T revealed the presence of a pac-type temperate bacteriophage, designated Φ20617, whose genomic organization and structure resemble those of temperate streptococcal bacteriophages. The prophage integrated at the 3'-end of the gene encoding the glycolytic enzyme enolase (eno), between eno and the lipoteichoic acid synthase-encoding gene ltaS, affecting their transcription. Comparative experiments c

SUBMITTER: Arioli S 

PROVIDER: S-EPMC6237837 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature