Fermentative Production of N-Methylglutamate From Glycerol by Recombinant Pseudomonas putida.

ABSTRACT: N-methylated amino acids are present in diverse biological molecules in bacteria, archaea and eukaryotes. There is an increasing interest in this molecular class of alkylated amino acids by the pharmaceutical and chemical industries. N-alkylated amino acids have desired functions such as higher proteolytic stability, enhanced membrane permeability and longer peptide half-lives, which are important for the peptide-based drugs, the so-called peptidomimetics. Chemical synthesis of N-methylated amino acids often is limited by incomplete stereoselectivity, over-alkylation or the use of hazardous chemicals. Here, we describe metabolic engineering of Pseudomonas putida KT2440 for the fermentative production of N-methylglutamate from simple carbon sources and mon

SUBMITTER: Mindt M 

PROVIDER: S-EPMC6237917 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature