Prevention of radiation-induced bystander effects by agents that inactivate cell-free chromatin released from irradiated dying cells.

ABSTRACT: Radiation-induced bystander effect (RIBE) is a poorly understood phenomenon wherein non-targeted cells exhibit effects of radiation. We have reported that cell-free chromatin (cfCh) particles that are released from dying cells can integrate into genomes of surrounding healthy cells to induce DNA damage and inflammation. This raised the possibility that RIBE might be induced by cfCh released from irradiated dying cells. When conditioned media from BrdU-labeled irradiated cells were passed through filters of pore size 0.22 µm and incubated with unexposed cells, BrdU-labeled cfCh particles could be seen to readily enter their nuclei to activate H2AX, active Caspase-3, NFκB, and IL-6. A direct relationship was observed with respect to activation of RIBE biomarkers and radiation dose in the ran

SUBMITTER: Kirolikar S 

PROVIDER: S-EPMC6238009 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature