MicroRNAs and their targets in cucumber shoot apices in response to temperature and photoperiod.

ABSTRACT:

Background

The cucumber is one of the most important vegetables worldwide and is used as a research model for study of phloem transport, sex determination and temperature-photoperiod physiology. The shoot apex is the most important plant tissue in which the cell fate and organ meristems have been determined. In this study, a series of whole-genome small RNA, degradome and transcriptome analyses were performed on cucumber shoot apical tissues treated with high vs. low temperature and long vs. short photoperiod.

Results

A total of 164 known miRNAs derived from 68 families and 203 novel miRNAs from 182 families were identified. Their 4611 targets were predicted using psRobot and TargetFinder, amongst which 349 were validated by degradome sequencing. Fourteen targets of six miRN

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6238408 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature