Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality.

ABSTRACT: Laminin-integrin interactions regulate various adhesion-dependent cellular processes. γ1C-Glu, the Glu residue in the laminin γ1 chain C-terminal tail, is crucial for the binding of γ1-laminins to several integrin isoforms. Here, we investigated the impact of γ1C Glu to Gln mutation on γ1-laminin binding to all possible integrin partners in vitro, and found that the mutation specifically ablated binding to α3, α6, and α7 integrins. To examine the physiological significance of γ1C-Glu, we generated a knock-in allele, Lamc1 ^EQ , in which the γ1C Glu to Gln mutation was introduced. Although Lamc1 ^EQ/EQ homozygotes developed into blastocysts and deposited laminins in their basement membranes, they died just after implantation because of

SUBMITTER: Kiyozumi D 

PROVIDER: S-EPMC6238537 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature