Restriction Fragment Length Polymorphism-based Genotyping of Toxoplasma gondii from Autopsy-Proven Cases of Acquired Immunodeficiency Syndrome-associated Cerebral Toxoplasmosis.

ABSTRACT:

Context

Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking.

Aims

The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India.

Settings and design

Archived tissues of CT were obtained postmortem from 25 acquired immunodeficiency syndrome patients between 2000 and 2014.

Subjects and methods

Direct amplification of eight different loci, namely, SAG1, 5'-3'SAG2, Alt. SAG2, SAG3, BTUB, GRA6, C22-8, and L358 followed by restriction fragment length polymorphism was used to genotype the parasite.

Results

The canonical Types I, II, or III were not found in our study. More th

SUBMITTER: Vijaykumar BR 

PROVIDER: S-EPMC6238572 | biostudies-literature | 2018 Oct-Dec

REPOSITORIES: biostudies-literature