Ultrasensitive Immunoassay for Simian Immunodeficiency Virus p27^CA.

ABSTRACT: Although effective for suppressing viral replication, combination antiretroviral treatment (cART) does not represent definitive therapy for HIV infection due to persistence of replication-competent viral reservoirs. The advent of effective cART regimens for simian immunodeficiency virus (SIV)-infected nonhuman primates (NHP) has enabled the development of relevant models for studying viral reservoirs and intervention strategies targeting them. Viral reservoir measurements are crucial for such studies but are problematic. Quantitative polymerase chain reaction (PCR) assays overestimate the size of the replication competent viral reservoir, as not all detected viral genomes are intact. Quantitative viral outgrowth assays measure replication competence, but they suffer from limited precision

SUBMITTER: Swanstrom AE 

PROVIDER: S-EPMC6238615 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature