Calcium-Infiltrated Biphasic Hydroxyapatite Scaffolds for Human Hematopoietic Stem Cell Culture.

ABSTRACT: Long-term in vitro expansion of hematopoietic stem cells (HSCs), while maintaining their functionality and multilineage differentiation potential, is still challenging. In this study, three-dimensional (3D) high-porosity hydroxyapatite (HA) foams have been designed to closely mimic the chemistry and physical structure of cancellous bone. Furthermore, calcium oxide was distributed in the HA ceramics to provide surface calcium ion release, hypothesizing that a local surface calcium gradient supports HSC localization and maintenance. Primary human HSCs and osteoblasts were cocultured for 6 weeks. Controls were cultured in two-dimensional dishes, while scaffold cultures were performed with calcium nitrate-infiltrated HA scaffolds and untreated HA scaffolds. Cells were analyzed for surface mark

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC6238654 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature