Emergence of tigecycline resistance in Escherichia coli co-producing MCR-1 and NDM-5 during tigecycline salvage treatment.

ABSTRACT:

Objective

Here, we report a case of severe infection caused by Escherichia coli that harbored mcr-1, bla _NDM-5, and acquired resistance to tigecycline during tigecycline salvage therapy.

Methods

Antimicrobial susceptibility testing, Southern blot hybridization, and complete genome sequence of the strains were carried out. The genetic characteristics of the mcr-1 and bla _NDM-5 plasmids were analyzed. The whole genome sequencing of mcr-1-containing plasmid was completed. Finally, putative single nucleotide polymorphisms and deletion mutations in the tigecycline-resistant strain were predicted.

Results

Three E. coli isolates were obtained from ascites, pleural effusion, and stool of a patient; they we

SUBMITTER: Wang Q 

PROVIDER: S-EPMC6239116 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature