T-cell Responses to <i>TP53</i> "Hotspot" Mutations and Unique Neoantigens Expressed by Human Ovarian Cancers.

ABSTRACT: Purpose: This was a study prospectively evaluating intratumoral T-cell responses to autologous somatic mutated neoepitopes expressed by human metastatic ovarian cancers.Patients and Methods: Tumor-infiltrating lymphocytes (TIL) were expanded from resected ovarian cancer metastases, which were analyzed by whole-exome and transcriptome sequencing to identify autologous somatic mutations. All mutated neoepitopes, independent of prediction algorithms, were expressed in autologous antigen-presenting cells and then cocultured with TIL fragment cultures. Secretion of IFNγ or upregulation of 41BB indicated a T-cell response.Results: Seven women with metastatic ovarian cancer were evaluated, and 5 patients had clear, dominant T-cell responses to mutated neoantigens, which were

SUBMITTER: Deniger DC 

PROVIDER: S-EPMC6239943 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature