Adenosine Triphosphate Release is Required for Toll-Like Receptor-Induced Monocyte/Macrophage Activation, Inflammasome Signaling, Interleukin-1β Production, and the Host Immune Response to Infection.

ABSTRACT:

Objectives

Monocytes and macrophages produce interleukin-1β by inflammasome activation which involves adenosine triphosphate release, pannexin-1 channels, and P2X7 receptors. However, interleukin-1β can also be produced in an inflammasome-independent fashion. Here we studied if this mechanism also involves adenosine triphosphate signaling and how it contributes to inflammasome activation.

Design

In vitro studies with human cells and randomized animal experiments.

Setting

Preclinical academic research laboratory.

Subjects

Wild-type C57BL/6 and pannexin-1 knockout mice, healthy human subjects for cell isolation.

Interventions

Human monocytes and U937 macrophages were treated with different inhibitors to study how purinergic signaling contributes to toll-li

SUBMITTER: Lee AH 

PROVIDER: S-EPMC6239954 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature