Real-time imaging of intracellular hydrogen peroxide in pancreatic islets.

ABSTRACT: A real-time method to measure intracellular hydrogen peroxide (H₂O₂) would be very impactful in characterizing rapid changes that occur in physiologic and pathophysiologic states. Current methods do not provide the sensitivity, specificity and spatiotemporal resolution needed for such experiments on intact cells. We developed the use of HyPer, a genetic indicator for H₂O₂ that can be expressed in the cytosol (cyto-HyPer) or the mitochondria (mito-HyPer) of live cells. INS-1 cells or islets were permeabilized and the cytosolic HyPer signal was a linear function of extracellular H₂O₂, allowing fluorescent cyto-HyPer signals to be converted into H₂O₂ concentrations. Glucose increased cytosolic H₂

SUBMITTER: Neal A 

PROVIDER: S-EPMC6240002 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature