The transient receptor potential melastatin 4 channel inhibitor 9-phenanthrol modulates cardiac sodium channel.

ABSTRACT:

Background and purpose

9-Phenanthrol, known as a specific inhibitor of the transient receptor potential melastatin 4 (TRMP4) channel, has been shown to modulate cardiac electrical activity and exert antiarrhythmic effects. However, its pharmacological effects remain to be fully explored. Here, we tested the hypothesis that cardiac sodium current inhibition contributes to the cardioprotective effect of 9-phenanthrol.

Experimental approach

Single ventricular myocytes (VMs) and Purkinje cells (PCs) were enzymatically isolated from rabbits. Arterially perfused rabbit wedge preparations were also used, and transmural electrocardiogram and endocardial action potentials (APs) were simultaneously recorded. Wild-type and mutated human recombinant SCN5A were expressed in HEK293 cells.

SUBMITTER: Hou JW 

PROVIDER: S-EPMC6240128 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature