Zinc finger protein 91 positively regulates the production of IL-1β in macrophages by activation of MAPKs and non-canonical caspase-8 inflammasome.

ABSTRACT:

Background and purpose

IL-1β is a cytokine of critical importance in inflammatory, infectious and autoimmune diseases. Zinc finger protein 91 (ZFP91) has been reported to be involved in multiple biological processes. Here, we identified a previously unknown role for ZFP91 in the production of biologically active IL-1β and investigated the underlying mechanisms of its effects.

Experimental approach

In vitro, the underlying mechanisms of ZFP91 at inhibiting the expression of IL-1β were investigated by ELISA, RT-PCR, Western blotting, immunoprecipitation and immunofluorescence assays. In vivo, colitis was induced by giving 4% dextran sulfate sodium (DSS) p.o. in drinking water for 5 days. Peritonitis was induced by injecting 700 μg alum i.p. for 12 h.

Key results

ZFP91 a

SUBMITTER: Mi C 

PROVIDER: S-EPMC6240129 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature