Taxonomic classification for microbiome analysis, which correlates well with the metabolite milieu of the gut.

ABSTRACT:

Background

16S rRNA gene amplicon sequencing analysis (16S amplicon sequencing) has provided considerable information regarding the ecology of the intestinal microbiome. Recently, metabolomics has been used for investigating the crosstalk between the intestinal microbiome and the host via metabolites. In the present study, we determined the accuracy with which 16S rRNA gene data at different classification levels correspond to the metabolome data for an in-depth understanding of the intestinal environment.

Results

Over 200 metabolites were identified using capillary electrophoresis and time-of-flight mass spectrometry (CE-TOFMS)-based metabolomics in the feces of antibiotic-treated and untreated mice. 16S amplicon sequencing, followed by principal component analysis (PCA) of

SUBMITTER: Wakita Y 

PROVIDER: S-EPMC6240276 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature