Differential effect of surgical manipulation on gene expression in normal breast tissue and breast tumor tissue.

ABSTRACT:

Background

Gene expression profiles of normal and tumor tissue reflect both differences in biological processes taking place in vivo and differences in response to stress during surgery and sample handling. The effect of cold (room temperature) ischemia in the time interval between surgical removal of the specimen and freezing is described in a few studies. However, not much is known about the effect of warm (body temperature) ischemia during surgery.

Methods

Three women with primary operable breast cancer underwent in situ biopsies from normal breast and tumor tissue prior to radical mastectomy. Ex vivo biopsies from normal and tumor tissue were collected immediately after surgical excision. The putative effects on gene expression of malignancy (tumor versus normal), surgic

SUBMITTER: Pedersen IS 

PROVIDER: S-EPMC6240321 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature