Microsatellite markers for Corybas (Orchidaceae) species in New Zealand.

ABSTRACT:

Premise of the study

Microsatellite markers were developed for New Zealand species of Corybas (Orchidaceae) to investigate population genetics and species delimitation.

Methods and results

From sequencing a total genomic DNA library (using Illumina MiSeq), we developed 22 microsatellite markers for C. obscurus. The di- and trinucleotide repeat loci were initially trialed on individuals representing seven Corybas taxa (C. "rimutaka," C. confusus, C. hypogaeus, C. macranthus, C. obscurus, C. trilobus, and C. walliae) and had one to eight alleles per locus. Twelve polymorphic markers were further tested on six Corybas populations from three of the seven taxa (C. obscurus, C. "rimutaka," a

SUBMITTER: Van Etten ML 

PROVIDER: S-EPMC6240450 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature