RPS15a Silencing Suppresses Cell Proliferation and Migration of Gastric Cancer.

ABSTRACT:

Purpose

Information on the possible role of the ribosomal protein S15a (RPS15a) in gastric cancer is scarce. The aim of this study was to evaluate the impact of RPS15a gene expression on the growth and cell cycle of gastric cancer cells in vitro and in vivo.

Materials and methods

RPS15a mRNA expression was examined in cancer tissues and their corresponding adjacent normal tissues of 40 gastric adenocarcinoma patients. Next, RPS15a was knocked down using a lentivirus-mediated RNA interference (short hairpin RNA) system in the gastric cancer cell line BGC823. The effect of RPS15a knockdown was examined using CCK-8 assay, cell scratch test, colony formation assay, and flow cytometry. Finally, in nude mice, a tumorigenicity test was performed, and the tumor volume

SUBMITTER: Shi D 

PROVIDER: S-EPMC6240561 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature