Pif1 helicase unfolding of G-quadruplex DNA is highly dependent on sequence and reaction conditions.

ABSTRACT: In addition to unwinding double-stranded nucleic acids, helicase activity can also unfold noncanonical structures such as G-quadruplexes. We previously characterized Pif1 helicase catalyzed unfolding of parallel G-quadruplex DNA. Here we characterized unfolding of the telomeric G-quadruplex, which can fold into antiparallel and mixed hybrid structures and found significant differences. Telomeric DNA sequences are unfolded more readily than the parallel quadruplex formed by the c-MYC promoter in K⁺ Furthermore, we found that under conditions in which the telomeric quadruplex is less stable, such as in Na⁺, Pif1 traps thermally melted quadruplexes in the absence of ATP, leading to the appearance of increased product formation under conditions in which the enzyme is prei

SUBMITTER: Byrd AK 

PROVIDER: S-EPMC6240867 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature