Allele-specific methylation of imprinted genes in fetal cord blood is influenced by cis-acting genetic variants and parental factors.

ABSTRACT:

Aim

To examine the effects of genetic variation, parental age and BMI on parental allele-specific methylation of imprinted genes in fetal cord blood samples.

Methodology

We have developed SNP genotyping and deep bisulphite sequencing assays for six imprinted genes to determine parental allele-specific methylation patterns in diploid somatic tissues.

Results

Multivariate linear regression analyses revealed a negative correlation of paternal age with paternal MEG3 allele methylation in fetal cord blood. Methylation of the maternal PEG3 allele showed a positive correlation with maternal age. Paternal BMI was positively correlated with paternal MEST allele methylation. In addition to parental origin, allele-specific methylation of most imprinted genes was largely dependen

SUBMITTER: Potabattula R 

PROVIDER: S-EPMC6240887 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature