Selective parallel G-quadruplex recognition by a NIR-to-NIR two-photon squaraine.

ABSTRACT: Fluorescence imaging probes for specific G-quadruplex (G4) conformations are of considerable interest in biomedical research. Herein, we present the synthesis and the binding properties of a new water-soluble near-infrared (NIR) amphiphilic squaraine dye (CAS-C1) which is capable of selective detection of parallel over non-parallel and non G4 topologies. The striking changes in its linear optical response upon binding to parallel G4s give rise to high fluorescence quantum yields (Φ _f ≈ 0.7) and one-photon molecular brightness in the far-red-NIR region. The outstanding recognition process of CAS-C1 for parallel G4s via end-stacking provides binding constants in the nanomolar regime (K _b = 10⁷ to 10⁸ M^-1

SUBMITTER: Grande V 

PROVIDER: S-EPMC6240894 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature